|Year : 2016 | Volume
| Issue : 1 | Page : 114
Comparative study on the effect of Plantago psyllium and Ocimum basilicum seeds on anthropometric measures in nonalcoholic fatty liver patients
Shahab-Aldin Akbarian1, Sedigheh Asgary2, Awat Feizi3, Bijan Iraj4, Gholamreza Askari1
1 Department of Community Nutrition, Food Security Research Center, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
2 Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Biostatistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran
4 Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
|Date of Submission||10-Aug-2016|
|Date of Acceptance||23-Aug-2016|
|Date of Web Publication||10-Oct-2016|
Department of Community Nutrition, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences, Hezar Jarib Street, Isfahan
Source of Support: None, Conflict of Interest: None
Clinical trial registration IRCT2014101811763N17
Background: Due to the attribution of fatty liver with some chronic diseases such as obesity, finding a way to control obesity can be useful for the management of fatty liver. This study was performed to assess the effects of Plantago psyllium (PP) and Ocimum basilicum (OB) on anthropometric measurements in people with hepatic steatosis.
Methods: All patients with nonalcoholic fatty liver disease (NAFLD) were enrolled in this four-arm parallel, randomized, and single blind trial. They randomly assigned into four groups receiving (1) OB 10 g/day; (2) PP 10 g/day; (3) mix of OB and PP 10 g/day; and (4) control group without placebo for 12 weeks. Anthropometric measurements were assessed during study baseline and after 12 weeks intervention. The data were analyzed using paired sample t-test for within group and analysis of covariance for between groups.
Results: In within group analysis, weight and body mass index show a significant reduction after 12 weeks intervention. In addition, soft lean mass and lean body mass were decreased in PP and mixed of PP and OB groups significantly; another group (OB) shows the same result for mass body fat. Although in intervention groups, we see considerable reduction, between group changes did not demonstrate the same consequences.
Conclusions: The results of this study showed that administration of OB, PP, or mix of them for 12 weeks does not affect any of the anthropometric measures in NAFLD.
Keywords: Fiber, mucilage, nonalcoholic fatty liver, obesity, Ocimum basilicum, Plantago psyllium, weight
|How to cite this article:|
Akbarian SA, Asgary S, Feizi A, Iraj B, Askari G. Comparative study on the effect of Plantago psyllium and Ocimum basilicum seeds on anthropometric measures in nonalcoholic fatty liver patients. Int J Prev Med 2016;7:114
|How to cite this URL:|
Akbarian SA, Asgary S, Feizi A, Iraj B, Askari G. Comparative study on the effect of Plantago psyllium and Ocimum basilicum seeds on anthropometric measures in nonalcoholic fatty liver patients. Int J Prev Med [serial online] 2016 [cited 2017 Sep 23];7:114. Available from: http://www.ijpvmjournal.net/text.asp?2016/7/1/114/191865
| Introduction|| |
Nonalcoholic fatty liver disease (NAFLD) as a silent killer with accumulating extra fat in hepatocytes more than 5% with no history of alcohol abuse, follows by creating inflammation, nonalcoholic steatohepatitis, which leads to liver fibrosis and finally liver cirrhosis.  High prevalence of NAFLD in diabetic patients and obese individuals showed the relationship between NAFLD and these chronic diseases which insulin resistance considered as the main reason.  Due to 25%-30% prevalence of total population,  finding solutions for NAFLD has an exclusive importance in public health.
Even though life styles modifications such as weight lose  are suggested, nutritional patterns changes are considered as important approaches in NAFLD management.  Simple carbohydrates, especially fructose and sucrose cause fat accumulation in body tissues such as liver.  Many studies assessed effect of nutrients such as Vitamin D, symbiotic and green tea as a solution. ,, Moreover, most NAFLD patients daily consume much lower fiber than required amounts. 
Plantago psyllium (PP) and Ocimum basilicum (OB) as two types of high fiber and mucilage (10%-15%),  plant seeds can consider as the key solution of NAFLD by decreasing appetite with occupying stomach and decreasing food glysemic index.
| Methods|| |
Present study is a four-arm parallel, randomized, and single blind trial conducted in Sedigheh Tahereh, Isfahan Endocrine and Metabolism Research Center in August 2014 to March 2015. We selected new cases of NAFLD at their first time referral to Fatty Liver Clinic.
Study population, eligibility and consent
We recruited men and women between 27 and 74 years old diagnosed with fatty liver ultrasonography, a well-established technique.  Individuals with steady using of fiber supplements, insulin injection, and drugs such as corticosteroids, anticonvulsants, and antiepileptic, also pregnant, lactating, hemochromatosis, and cirrhosis patients were excluded. All participants filled informed consent before involved in the study.
Intervention and monitoring
We supplied PP and OB seeds from the center of Iran, Isfahan, which packaged in 2 5 g parts for daily (10 g) usage. Each pack should be eaten 20-30 min before lunch and dinner with only a glass of water and no additional sugar or even artificial sweeteners for 3 months. We followed patient with weekly texting and monthly calling.
Mucilage of PP and OB absorb water and get a sticky and viscose condition which leads to unpleasant feeling, so we lost participants and mentioned in [Figure 1].
Plantago psyllium and Ocimum basilicum components
We measured macronutrients and fiber components of PP and OB. Crude fiber by assessment tool (PP: 33 and OB: 31%) and dietary fiber by Association of Official Analytical Chemists (AOAC) 199/43 method (PP: 80 and OB: 59%),  carbohydrate by Fehling method (PP: 0.5 and OB: 1%),  protein by Kjeldahl method (PP: 18 and OB: 25%),  and fat by Soksele method  (PP: 1.5 and OB: 20%) were measured at the beginning of study.
We assessed all anthropometric measurements at the beginning and end of the study. Heights were measured with a standard tape and without shoes to the nearest 0.5 cm. Weight to the nearest 0.1 kg, percent body fat (PBF %), body mass index (BMI), soft lean mass (SLM kg), mass body fat (MBF kg), and also both MBF and SLM for trunk were all measured by Body Composition Analyzer device (Janex Medical Company, ioi 353 model). Then, 3-day food records of 2 usual days and 1 day off and a 2-day physical activity record were taken. Physical activity was calculated as a metabolic equivalent task minute per day spent on all activities with accurate each activity time.
Sample size, randomization, and blinding
We allocated 120 participants in four groups with permuted block randomization of size 4. We provided a list of randomized numbers that had 4 numbers in each group then, one blinded staff of Fatty Liver Clinic put the selected patients in four different groups (1: OB, 2: PP, 3: OB and PP mixed, and 4: Control) secretly. Because patients should pour the seeds into a glass of water, they could not be blinded. But, laboratory staff, one ultrasound specialist, and who explained the records and consumption structures of the intervention were blinded in this study.
Quantitative and qualitative variables were expressed as mean ± standard error (or median [inter quartile range]) and frequency (percentage). Normality of quantitative variables was evaluated using Kolmogorov-Smirnov test and Q-Q plot. Nonnormal variables were subjected to logarithmic transformation. Within group analysis for quantitative variables was conducted using paired samples t-test. Within group analysis for quantitative variables was conducted using paired samples t-test. For between group comparisons analysis of covariance (ANCOVA) was used and baseline values of dependent variables, age and energy intake were adjusted. Chi-square or Fisher exact tests (if appropriate) were used for comparing the qualitative variables between groups. Statistical analyses carried out by the use of Statistical Package for the Social Sciences (SPSS) version 16 (SPSS, Inc, Chicago, IL, USA).
| Results|| |
Eight participants could not tolerate PP and OB because of constipation, nausea or stomach-ache and were excluded from our study. Moreover, 42 patients that were involved to exclusion criteria during the study or they did not want to continue were eliminated. There were no significant differences regarding sex distribution, smoking condition, education status, and body weight in four groups at baseline. However, age was the only factor that was significantly different among groups which was adjusted in our analysis. No significant difference of receiving pill was observed among groups. [Table 1] is showing all participants demographic information.
First of all, we compared before and after anthropometric measurements in different groups separately that some of them had been significantly decreased. Weight (P = 0.02), BMI (P = 0.01), and MBF (P = 0.04) in OB group were significantly reduced. Moreover, weight (P = 0.00), BMI, SLM, and lean body mass (LBM) (P = 0.01) were lowered in PP group. In PP and OB mixed group, we saw a similar result to the last group. Weight, BMI, SLM, and LBM were significantly different while, SLM of trunk was marginally significant. Similarly, control group data showed considerable reduction in weight and BMI P = 0.00 and 0.02, respectively, we examined among group changes as more important aim in order to reach reliable consequences of our intervention that we did not see any significant effects among groups [Table 2].
In addition, we averaged out a 3-day food and a 2-day record in order to controlled confounding such as a total dietary, total fiber, insoluble fiber, and soluble fiber intake [Table 3] and also, physical activity [Table 1]. None of the mean dietary confounding factors and mean of physical activity was significantly different among four groups.
| Discussion|| |
This study illustrates a 10 g increasing of PP, OB, or mix of them have no effects on weight, BMI, % PBF, or SLM during 12 weeks. Previous studies have recommended that increased fiber intake up to Recommended Dietary Allowance (RDA) amount (38 for men and 25 g for women  leads to increased postmeal satiety.  Moreover, the gut hormone, ghrelin, gastrointestinal peptide, and glucagon-like peptide (GLP-1) were increased. Ghrelin can suppress appetite and GLP-1 can enhance postprandial satiety time. 
However, the effects of fiber on weight loss are controversial. Similar to our study, no protective effect of PP on weight change did not observed in 36 diabetic patients.  Likewise, when soluble fiber was compared against insoluble fiber neither of them have cause reduction in weight. 
In contrast, some documents showed positive outcomes of soluble fiber in anthropometric characters. High dose of PP (36 g daily) demonstrated considerable decrease in weight, BMI, and, especially % body fat either with a diet modification or without that.  Similarly, both soluble (24 g PP) and insoluble (28 g wheat bran) elucidated a significantly weight loss after 6 weeks, while the participants had gone on a modified diet. 
As other studies, our study includes some limitations inevitably. Some used drugs by patients to improve liver status, might fade the effects of PP and OB on anthropometric measures. Our participants were new cases of NAFLD who may have gone on a diet unconsciously.
The aim of present study was to demonstrate protective effect of PP and OB mixture on weight reduction but it was not observed which can be due to the low-dose of soluble fiber used in present study.
In a similar meta-analysis, the effect of guar gum on body weight was evaluated rigorously. As a consequence, when 11 randomized controlled trials were put together, no significant robust effect of guar gum consumption on weight loss was observed.  Although most animal studies showed a significant effect of fiber on weight reduction,  human studies have yielded inconsistent inferences. , Likewise, it is shown in another systematic review that soluble fiber has no effect on weight loss.  However, it should be noted in our study and other similar studies, participant's baseline weight was normal or overweight which may have precluded the expected results. This study has been registered both in Iranian registry clinical trial (IRCT2014101811763N17) and Isfahan University of Medical Sciences (393583).
| Conclusions|| |
This study indicated that supplementation with 10 mg/day PP, 10 mg/day OB, and 10 mg/day PP and OB mixed for 12 weeks did not improve weight, BMI, SLM, LBM, MBF, and PBF significantly. Further trials should be done in this area with higher dose of PP and OB and also, in bigger population which draw a clear link between anthropometric measures and fiber supplementation.
This study was funded by Isfahan University of Medical Sciences and Sedigheh Tahereh, Isfahan Endocrine and Metabolism Research Center was involved in data collection.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Milic S, Lulic D, Štimac D. Non-alcoholic fatty liver disease and obesity: Biochemical, metabolic and clinical presentations. World J Gastroenterol 2014;20:9330-7.
Gariani K, Philippe J, Jornayvaz FR. Non-alcoholic fatty liver disease and insulin resistance: From bench to bedside. Diabetes Metab 2013;39:16-26.
Pascale A, Pais R, Ratziu V. An overview of nonalcoholic steatohepatitis: Past, present and future directions. J Gastrointestin Liver Dis 2010;19:415-23.
Ueno T, Sugawara H, Sujaku K, Hashimoto O, Tsuji R, Tamaki S, et al.
Therapeutic effects of restricted diet and exercise in obese patients with fatty liver. J Hepatol 1997;27:103-7.
Fan JG, Cao H ×. Role of diet and nutritional management in non-alcoholic fatty liver disease. J Gastroenterol Hepatol 2013;28 Suppl 4:81-7.
Nomura K, Yamanouchi T. The role of fructose-enriched diets in mechanisms of nonalcoholic fatty liver disease. J Nutr Biochem 2012;23:203-8.
Foroughi M, Maghsoudi Z, Ghiasvand R, Iraj B, Askari G. Effect of Vitamin D supplementation on C-reactive protein in patients with nonalcoholic fatty liver. Int J Prev Med 2014;5:969-75.
Pezeshki A, Safi S, Feizi A, Askari G, Karami F. The effect of green tea extract supplementation on liver enzymes in patients with nonalcoholic fatty liver disease. Int J Prev Med 2016;7:28.
Asgharian A, Askari G, Esmailzade A, Feizi A, Mohammadi V. The effect of symbiotic supplementation on liver enzymes, C-reactive protein and ultrasound findings in Patients with non-alcoholic fatty liver disease: A Clinical Trial. Int J Prev Med 2016;7:59.
Ferolla SM, Ferrari TC, Lima ML, Reis TO, Tavares-Jr WC, Couto OF, et al.
Dietary patterns in Brazilian patients with nonalcoholic fatty liver disease: A cross-sectional study. Clinics (Sao Paulo) 2013;68:11-7.
Koocheki A, Tabrizi L, Mahallati MN. The Effects of irrigation intervals and manure on quantitative and qualitative characteristics of Plantago ovata
and Plantago psyllium
. Asian J Plant Sci 2007;6:1229-34.
Lee SS, Park SH. Radiologic evaluation of nonalcoholic fatty liver disease. World J Gastroenterol 2014;20:7392-402.
Firestone D, Horwitz W. IUPAC gas chromatographic method for determination of fatty acid composition: Collaborative study. J Assoc Off Anal Chem 1979;62:709-21.
Chaira N, Ferchichi A, Mrabet A, Sghairoun M. Chemical composition of the flesh and the pit of date palm fruit and radical scavenging activity of their extracts. Pak J Biol Sci 2007;10:2202-7.
Vinklárková B, Chromý V, Šprongl L, Bittová M, Rikanová M, Ohnútková I, et al.
The Kjeldahl method as a primary reference procedure for total protein in certified reference materials used in clinical chemistry. II. Selection of direct Kjeldahl analysis and its preliminary performance parameters. Crit Rev Anal Chem 2015;45:112-8.
Mehdi B, Armin K, Reza S. Changes in nutritional factors of Freezed Kutum (Rutilus frisii kutum
). World J Zool 2008;2:51-3.
Trumbo P, Schlicker S, Yates AA, Poos M; Food and Nutrition Board of the Institute of Medicine, The National Academies. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein and amino acids. J Am Diet Assoc 2002;102:1621-30.
Wanders AJ, Mars M, Borgonjen-van den Berg KJ, de Graaf C, Feskens EJ. Satiety and energy intake after single and repeated exposure to gel-forming dietary fiber: Post-ingestive effects. Int J Obes (Lond) 2014;38:794-800.
Karhunen LJ, Juvonen KR, Flander SM, Liukkonen KH, Lähteenmäki L, Siloaho M, et al.
A psyllium fiber-enriched meal strongly attenuates postprandial gastrointestinal peptide release in healthy young adults. J Nutr 2010;140:737-44.
Ziai SA, Larijani B, Akhoondzadeh S, Fakhrzadeh H, Dastpak A, Bandarian F, et al.
Psyllium decreased serum glucose and glycosylated hemoglobin significantly in diabetic outpatients. J Ethnopharmacol 2005;102:202-7.
Vuksan V, Jenkins AL, Rogovik AL, Fairgrieve CD, Jovanovski E, Leiter LA. Viscosity rather than quantity of dietary fibre predicts cholesterol-lowering effect in healthy individuals. Br J Nutr 2011;106:1349-52.
Pal S, Khossousi A, Binns C, Dhaliwal S, Ellis V. The effect of a fibre supplement compared to a healthy diet on body composition, lipids, glucose, insulin and other metabolic syndrome risk factors in overweight and obese individuals. Br J Nutr 2011;105:90-100.
Anderson JW, Riddell-Mason S, Gustafson NJ, Smith SF, Mackey M. Cholesterol-lowering effects of psyllium-enriched cereal as an adjunct to a prudent diet in the treatment of mild to moderate hypercholesterolemia. Am J Clin Nutr 1992;56:93-8.
Pittler MH, Ernst E. Guar gum for body weight reduction: Meta-analysis of randomized trials. Am J Med 2001;110:724-30.
Levrat MA, Favier ML, Moundras C, Rémésy C, Demigné C, Morand C. Role of dietary propionic acid and bile acid excretion in the hypocholesterolemic effects of oligosaccharides in rats. J Nutr 1994;124:531-8.
Pittler MH, Ernst E. Dietary supplements for body-weight reduction: A systematic review. Am J Clin Nutr 2004;79:529-36.
[Table 1], [Table 2], [Table 3]