International Journal of Preventive Medicine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 8  |  Issue : 1  |  Page : 18-

Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells


Samira Negahdari1, Hamid Galehdari1, Mahnaz Kesmati2, Anahita Rezaie3, Gholamreza Shariati4 
1 Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Department of Biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Department of Veterinary, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Department of Medical Genetics, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Correspondence Address:
Hamid Galehdari
Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz
Iran

Background: Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such as Adiantum capillus-veneris, Commiphora molmol, Aloe vera, and henna and one mixture of them were used to treatment of normal mouse skin fibroblasts. Methods: Cytotoxic effects of each extract and their mixture were assessed on mouse skin fibroblasts cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We performed migration assays to assess migration properties of mouse skin fibroblasts cells in response to the extracts. Changes in the gene expression of the Tgf β 1and Vegf-A genes were monitored by real-time polymerase chain reaction. Results: A. capillus-veneris, C. molmol and henna extract improved the expression of Tgf β 1 gene. All used extracts upregulated the expression of Vegf-A gene and promoted the migration of mouse fibroblast cells in vitro. Conclusions: The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgf β 1 and Vegf-A genes in fibroblast cells treated with extracts.


How to cite this article:
Negahdari S, Galehdari H, Kesmati M, Rezaie A, Shariati G. Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells.Int J Prev Med 2017;8:18-18


How to cite this URL:
Negahdari S, Galehdari H, Kesmati M, Rezaie A, Shariati G. Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells. Int J Prev Med [serial online] 2017 [cited 2017 Jul 24 ];8:18-18
Available from: http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2017;volume=8;issue=1;spage=18;epage=18;aulast=Negahdari;type=0