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  Indian J Med Microbiol
 

Figure 3: Dihydroartemisinin-dependent depletion of Δψ m (a-c) and increased reactive oxygen species generation (d and e). Following treatment with different concentration (0.1–100 μM) of dihydroartemisinin in the presence and absence of N-acetylcysteine (1 mM) for 48 h, cells were loaded with JC-1dye and fluorescence ratio measured. (c) Effect of time in Δψm, cells were treated with 20 μ M of dihydroartemisinin in a time intervals between 0-60 h. Following treatment, EJ-138 (d) and HTB-9 (e) cells were loaded with dichlorofluorescin diacetate and fluorescence was measured

Figure 3: Dihydroartemisinin-dependent depletion of <b>Δψ</b> m (a-c) and increased reactive oxygen species generation (d and e). Following treatment with different concentration (0.1–100 μM) of dihydroartemisinin in the presence and absence of N-acetylcysteine (1 mM) for 48 h, cells were loaded with JC-1dye and fluorescence ratio measured. (c) Effect of time in Δψm, cells were treated with 20 <b>μ</b> M of dihydroartemisinin in a time intervals between 0-60 h. Following treatment, EJ-138 (d) and HTB-9 (e) cells were loaded with dichlorofluorescin diacetate and fluorescence was measured