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  Indian J Med Microbiol
 

Figure 2: Chlorella sp. (CLC) supplementation effect on liver function marker enzymes in the mice hepatic tissue of administrated with APAP. GOT and GPT activities were measured according to the ELISA protocol of commercially available kit. All the groups were sacrificed after 4 weeks to liver sample to determine (a) GOT and (b) GPT activities in hepatic tissue. All of the values were expressed as the mean ± SD from 6 mice of each group. *P < 0.05, significant difference compared with control.#P < 0.05, significant difference compared with APAP-induced liver injury. All mice were sacrificed after 4 weeks and their livers isolated and homogenated for hepatic (c) SOD and (d) CAT activities determination. The SOD activity is standardized using the cytochrome c and xanthine oxidase coupled assay. The CAT activity is expressed in the equation showed as follows: k/mg protein = 2.3/(t2-t1) × log (A1/A2), where k is the first-order reaction rate constant, t is the time over which the decrease of H2O2due to CAT activity was measured 30 s, and A1/A2is the optical density at times 0 and 30 s, respectively. All of the values were expressed as the mean ± SD from 6 mice of each group. *P < 0.05, significant difference compared with control.#P < 0.05, significant difference compared with APAP-induced liver injury.(e) MDA is a marker for oxidative stress results from lipid peroxidation of polyunsaturated fatty acids. The determination of hepatic lipid peroxidation at the absorbance at 532 nm. All of the values were expressed as the mean ± SD from 6 mice of each group.*P < 0.05, significant difference compared with control.#P < 0.05, significant difference compared with APAP-induced liver injury. (f). The APAP, SM, 0.2% CLC, 0.5% CLC, and 1% CLC groups were administrated with 200 mg/kg APAP, and the normal control was administrated with PBS as placebo twice a week for 4 weeks. The Sm was fed with the chow diet containing 0.1% Silymarin (w/w). The group 0.2% CLC, 0.5% CLC, and 1% CLC were fed with the chow diet containing 0.2%, 0.5%, and 1% of CLC (w/w), respectively. All mice were sacrificed and the liver was removed, fixed, and embedded in paraffin. Histopathological photomicrographs of mouse livers of 6 groups stained with hematoxylin and eosin (H and E,200×). Collagen release is expressed by black arrows. Inflammatory cell infiltration is expressed by brown arrows

Figure 2: <i>Chlorella sp</i>. (CLC) supplementation effect on liver function marker enzymes in the mice hepatic tissue of administrated with APAP. GOT and GPT activities were measured according to the ELISA protocol of commercially available kit. All the groups were sacrificed after 4 weeks to liver sample to determine (a) GOT and (b) GPT activities in hepatic tissue. All of the values were expressed as the mean ± SD from 6 mice of each group. *<i>P</i> < 0.05, significant difference compared with control.<sup>#</sup><i>P</i> < 0.05, significant difference compared with APAP-induced liver injury. All mice were sacrificed after 4 weeks and their livers isolated and homogenated for hepatic (c) SOD and (d) CAT activities determination. The SOD activity is standardized using the cytochrome c and xanthine oxidase coupled assay. The CAT activity is expressed in the equation showed as follows: k/mg protein = 2.3/(t<sub>2</sub>-t<sub>1</sub>) × log (A<sub>1</sub>/A<sub>2</sub>), where k is the first-order reaction rate constant, t is the time over which the decrease of H<sub>2</sub>O<sub>2</sub>due to CAT activity was measured 30 s, and A<sub>1</sub>/A<sub>2</sub>is the optical density at times 0 and 30 s, respectively. All of the values were expressed as the mean ± SD from 6 mice of each group. *<i>P</i> < 0.05, significant difference compared with control.<sup>#</sup><i>P</i> < 0.05, significant difference compared with APAP-induced liver injury.(e) MDA is a marker for oxidative stress results from lipid peroxidation of polyunsaturated fatty acids. The determination of hepatic lipid peroxidation at the absorbance at 532 nm. All of the values were expressed as the mean ± SD from 6 mice of each group.*<i>P</i> < 0.05, significant difference compared with control.<sup>#</sup><i>P</i> < 0.05, significant difference compared with APAP-induced liver injury. (f). The APAP, SM, 0.2% CLC, 0.5% CLC, and 1% CLC groups were administrated with 200 mg/kg APAP, and the normal control was administrated with PBS as placebo twice a week for 4 weeks. The Sm was fed with the chow diet containing 0.1% Silymarin (w/w). The group 0.2% CLC, 0.5% CLC, and 1% CLC were fed with the chow diet containing 0.2%, 0.5%, and 1% of CLC (w/w), respectively. All mice were sacrificed and the liver was removed, fixed, and embedded in paraffin. Histopathological photomicrographs of mouse livers of 6 groups stained with hematoxylin and eosin (H and E,200×). Collagen release is expressed by black arrows. Inflammatory cell infiltration is expressed by brown arrows