|Year : 2016 | Volume
| Issue : 1 | Page : 40
Low serum Vitamin C status among pregnant women attending antenatal care at general hospital Dawakin Kudu, Northwest Nigeria
Emmanuel Ajuluchukwu Ugwa1, Elemi Agbor Iwasam2, Matthew Igwe Nwali3
1 Department of Obstetrics and Gynaecology, Federal Medical Centre, Birnin Kudu, Jigawa, Nigeria
2 Department of Community Medicine, University of Calabar Teaching Hospital, Calabar, Cross River, Nigeria
3 Department of Obstetrics and Gynaecology, Federal Teaching Hospital, Abakaliki, Ebonyi, Nigeria
|Date of Submission||09-Apr-2015|
|Date of Acceptance||14-Dec-2015|
|Date of Web Publication||11-Feb-2016|
Emmanuel Ajuluchukwu Ugwa
Department of Obstetrics and Gynaecology, Federal Medical Centre, Birnin Kudu, Jigawa
Source of Support: None, Conflict of Interest: None
Background: Vitamin C levels are low in pregnancy. The purpose of this study was to determine serum Vitamins C levels among pregnant women attending antenatal care at a General Hospital in Dawakin Kudu, Kano, and this can help further research to determine the place of Vitamin C supplementation in pregnancy.
Methods: This was a prospective study of 400 pregnant women who presented for antenatal care in General Hospital Dawakin Kudu, Kano, Nigeria. Research structured questionnaire was administered to 400 respondents. Determination of serum Vitamin C was done using appropriate biochemical methods.
Results: Vitamin C deficiency was found in 79.5% of the participants. The values for Vitamin C were 0.20 ± 0.18 mg/dl during the first trimester, 0.50 ± 0.99 mg/dl in the second trimester, and 0.35 ± 0.36 mg/dl in the third trimester and P = 0.001.
Conclusions: There is a significant reduction in the serum Vitamins C concentration throughout the period of pregnancy with the highest levels in the second trimester. Therefore, Vitamin C supplementation is suggested during pregnancy, especially for those whose fruit and vegetable consumption is inadequate.
Keywords: North-west Nigeria, pregnant women, Vitamin C
|How to cite this article:|
Ugwa EA, Iwasam EA, Nwali MI. Low serum Vitamin C status among pregnant women attending antenatal care at general hospital Dawakin Kudu, Northwest Nigeria. Int J Prev Med 2016;7:40
|How to cite this URL:|
Ugwa EA, Iwasam EA, Nwali MI. Low serum Vitamin C status among pregnant women attending antenatal care at general hospital Dawakin Kudu, Northwest Nigeria. Int J Prev Med [serial online] 2016 [cited 2021 Oct 16];7:40. Available from: https://www.ijpvmjournal.net/text.asp?2016/7/1/40/176166
| Introduction|| |
Vitamin C, also known as ascorbic acid, like other vitamins is an organic substance, which is required by the body in small amounts to maintain life and health.  It acts as a catalyst in the formation of hormones, enzymes, blood cells, neurotransmitters, and genetic materials.  It is also essential to complete the metabolism of carbohydrates, proteins, and fats. The body's need for Vitamin C is met by diet, especially fruits. Vitamin C has antioxidant properties and as such either blocks the initiation of free radical formation or inactivate (scavenge) free radical. 
Vitamin C levels in the third trimester has been shown to be lower than the levels in the first and second trimester due to physiological changes in pregnancy leading to hemodilution  and deficiency of Vitamin C may be associated with some complications in pregnancy. 
Vitamin C supplementation has been shown in some studies to reduce the risk of premature rupture of placental membranes and premature births, gestational hypertension, intrauterine growth retardation, and gestational diabetes in addition to other health benefits. ,,,, Furthermore, because Vitamin C enhances iron absorption, , the low serum ascorbate can lead to a decrease in the absorption and subsequent utilization of iron which is required for the proper maintenance of pregnancy and fetal growth.
Data from Nigeria on Vitamin C status among pregnant women are few. The purpose of this study was to determine serum Vitamins C levels among pregnant women attending antenatal care at a General Hospital in Dawakin Kudu, Kano, Nigeria, and this can help further research to determine the place of Vitamin C supplementation in pregnancy.
| Methods|| |
This was a prospective study of 400 pregnant women at various maternal ages, gestational ages and parities done between 31 November 2009 and 30 March 2011. Ethical approval for the research was obtained from General Hospital, Dawakin Kudu, Kano, Nigeria. Informed consent was obtained from the participants.
Dawakin Kudu is a rural community in Kano State, Nigeria, and about 10 km from Kano, the commercial nerve center of Northern Nigeria. Most of the women are homemakers.
Research structured questionnaire was administered to 400 respondents randomly selected and this showed various sociodemographic indices.
Documented prevalence of 32.26% (20 out of 62) from previous study was used.  This study was chosen because it was done in a similar setting in Nigeria. The sample size was rounded up to 450 pregnant women to account for dropout. Participants with confounders such as hypertensive diseases and diabetes mellitus were excluded.
Whole blood sample (5 ml) was collected from the 400 participants and drawn directly into a plain blood sample container. Centrifugation at 2500-3000 rpm for 5 min was done to obtain plasma. They were labeled and covered with aluminum foils. Storage was in a deep freezer for 1 month. Determination of serum Vitamin C level, according to Roe and Kuether, 1943,  and normal range was 0.5-2 mg/dl.
Data obtained were analyzed using SPSS version 17 statistical software (SPSS Inc., Chicago, IL, USA). Descriptive statistics was used for quantitative variables. Mean serum Vitamins C concentrations between trimesters were compared using one-way ANOVA and P < 0.05 was considered statistically significant.
| Results|| |
Results are shown in [Table 1] [Table 2] [Table 3]. Out of 450 participants, 400 completed the study. Participants who could not be followed-up or who developed confounders such as hypertensive diseases or diabetes mellitus were dropped. Majority of the women were aged 20-39 years with a mean of 23.67 ± 6.11. Most were in the 1-4 parity range. Seventy-nine and half percentage (79.5%) of the women had Vitamin C deficiency. The values for Vitamin C were 0.20 ± 0.18 mg/dl during the first trimester, 0.50 ± 0.99 in the second trimester, and 0.35 ± 0.36 mg/dl in the third trimester. The differences between the vitamins from one trimester to the other were statistically significant (P < 0.05).
| Discussion|| |
The present study reports that majority of the women were aged 20-39 years and a mean of 23.67 ± 6.11 was reported. The previous study reported mean age of 27.2 ± 6.5 years.  The association between the status of Vitamin C and maternal age has been studied, and the highest levels were found among the age group 22-25 years and the lowest levels in the age group 34-37 years. 
Majority of the women in this study were in the 1-4 parity range. Some local studies have reported that the serum Vitamin C levels decrease with parity because multiparous women in our environment may have depleted vitamin storage from frequent pregnancies at short intervals. 
The present study reports low serum Vitamin C levels among this cohort of pregnant women and this was significant across the three trimesters. This study reports a prevalence of Vitamin C deficiency of 79.5%. A previous study from the northwestern part of Nigeria reported a lower prevalence of 32.3%.  This difference may be related to the high parity, inadequate nutrition, and nutritional taboos among Northern Nigeria pregnant women. A similar prevalence of 75% was earlier reported from a rural setting in India.  Similar studies found significant reduction in serum Vitamin C in pregnant Nigerians. , Contrary to other studies, plasma Vitamin C decline during pregnancy did not follow a linear trend with time. , Vitamin C deficiency may be accounted partly by the physiological hemodilution of pregnancy, inadequate intake, and the use of Vitamin C to combat the oxidative stress of pregnancy. 
This study has shown deficient Vitamin C status among pregnant women attending antenatal clinic at General Hospital Dawakin Kudu, Kano, and there is a significant reduction across trimesters. Although data on Vitamin C supplementation in pregnancy are conflicting, ,, Vitamin C supplementation can be suggested during pregnancy, especially for those whose fruit and vegetable consumption is inadequate because of aforementioned benefits. Vitamin C supplementation could also help to prevent the development of such complications of pregnancy such as gestational hypertension, intrauterine growth retardation, and gestational diabetes, conditions all known to be associated with high levels of oxidative stress.  Studies from less-developed economies where Vitamin C deficiency among pregnant mothers is prevalent showed significant benefit of Vitamin C supplementation during pregnancy in reducing low birth weight and small for gestational age births. , Vitamin C may be protective against development of preeclampsia. , It has also been shown that supplementation with Vitamins C and E after preterm premature rupture of membranes (PPROM) is associated with a statistically significant increase in difference in the number of days before delivery.  However, another study was of the contrary and does not support Vitamin C use for management or prevention of PPROM. 
Decision as to which micronutrients are of greatest concern in developing countries requires a more systematic and comprehensive approach. 
| Conclusions|| |
There is a significant reduction in the serum Vitamins C concentration throughout the period of pregnancy with the highest levels in the second trimester Therefore, Vitamin C supplementation is suggested during pregnancy, especially for those whose fruit and vegetable consumption are inadequate.
The author acknowledges the technical support provided by the laboratory staff of General Hospital Dawakin Kudu, during the preparation of this manuscript.
Financial support and sponsorship
Conflicts of interest
There are no conflict of interest.
| References|| |
Klufio CA. Nutrition in pregnancy. In: Kwawukume EY, Emuveyan EE, editors. Comprehensive Obstetrics in the Tropics. Dansoman: Asante and Hittcher Limited; 2002. p. 21-3.
Halliwell B. How to characterize a biological antioxidant radicals. Res Commun 1990;9:1-30.
Yoshioka T. Vitamins in pregnancy. Nihon Rinsho 1999;57:2381-4.
Therieko PA, Ettes SX. Plasma ascorbic acid levels in Nigerian mothers and newborn. J Trop Pediatr 1981;27:263-6.
Casanueva E, Viteri FE. Iron and oxidative stress in pregnancy. J Nutr 2003;133 5 Suppl 2:1700S-8S.
Frei B, England L, Ames BN. Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci U S A 1989;86:6377-81.
Rumbold A, Crowther CA. Vitamin C supplementation in pregnancy. Cochrane Database Syst Rev 2005;18:CD004072.
Casanueva E, Ripoll C, Meza-Camacho C, Coutiño B, Ramírez-Peredo J, Parra A. Possible interplay between Vitamin C deficiency and prolactin in pregnant women with premature rupture of membranes: Facts and hypothesis. Med Hypotheses 2005;64:241-7.
Osaikhuwuomwan JA, Okpere EE, Okonkwo CA, Ande AB, Idogun ES. Plasma Vitamin C levels and risk of preterm prelabour rupture of membranes. Arch Gynecol Obstet 2011;284:593-7.
Sharma DC, Mathur R. Correction of anemia and iron deficiency in vegetarians by administration of ascorbic acid. Indian J Physiol Pharmacol 1995;39:403-6.
Whitney EA, Hamilton EM. Understanding Nutrition. 3 rd
ed. Minnesota: West Publishing Company; 1984.
Ariyo O, Keshinro OO. serum ascorbic acid levels in the third trimester of pregnancy and ascorbate content of maternal breast milk. Cont J Med Res 2012;6:30-6.
Roe JH, Kuether CA. The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative; J Biol Chem 1943;147:399-407.
Sylvester IE, Paul A. Effects of socio demographic factors on plasma ascorbic acid and alpha tocopherol antioxidants during pregnancy. Pak J Med Sci 2009;25:755-9.
Ejezie EE, Onwusi EA, Nwagha UI. Some biochemical markers of oxidative stress in pregnant Nigerian women. Trop J Obstet Gynaecol 2004;21:122-4.
Chiplonkar SA, Agte VV, Mengale SS, Tarwadi KV. Are lifestyle factors good predictors of retinol and Vitamin C deficiency in apparently healthy adults? Eur J Clin Nutr 2002;56:96-104.
Uchenna IN, Fedilis EE. Serum ascorbic acid level during pregnancy in Enugu, Nigeria. J Col Med 2005;10:43-5.
Ugwa E, Gwarzo M, Ashimi A. Oxidative stress and antioxidant status of pregnant rural women in north-west Nigeria: Prospective cohort study. J Matern Fetal Neonatal Med 2015;28:544-7.
Stark JM. Inadequate reducing systems in pre-eclampsia: A complementary role for Vitamins C and E with thioredoxin-related activities. BJOG 2001;108:339-43.
Kontic-Vucinic O, Terzic M, Radunovic N. The role of antioxidant vitamins in hypertensive disorders of pregnancy. J Perinat Med 2008;36:282-90.
Holmes VA, McCance DR. Could antioxidant supplementation prevent pre-eclampsia? Proc Nutr Soc 2005;64:491-501.
Chappell LC, Seed PT, Kelly FJ, Briley A, Hunt BJ, Charnock-Jones DS, et al.
Vitamin C and E supplementation in women at risk of preeclampsia is associated with changes in indices of oxidative stress and placental function. Am J Obstet Gynecol 2002;187:777-84.
Olayaki LA, Ajao SM, Jimoh GA, Aremu IT, Soladoye AO. Effect of Vitamin C on malondialdehyde (MDA) in pregnant Nigerian women. J Basic Appl Sci 2008;4:105-8.
Bodnar LM, Tang G, Ness RB, Harger G, Roberts JM. Periconceptional multivitamin use reduces the risk of preeclampsia. Am J Epidemiol 2006;164:470-7.
Borna S, Borna H, Daneshbodie B. Vitamins C and E in the latency period in women with preterm premature rupture of membranes. Int J Gynaecol Obstet 2005;90:16-20.
Gungorduk K, Asicioglu O, Gungorduk OC, Yildirim G, Besimoglu B, Ark C. Does Vitamin C and Vitamin E supplementation prolong the latency period before delivery following the preterm premature rupture of membranes? A randomized controlled study. Am J Perinatol 2014;31:195-202.
Hauth JC, Clifton RG, Roberts JM, Spong CY, Myatt L, Leveno KJ, et al
. Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) Maternal-Fetal Medicine Units Network (MFMU). Vitamin C and E supplementation to prevent spontaneous preterm birth: A randomized controlled trial. Obstet Gynecol 2010;116:653-8.
Swaney P, Thorp J, Allen I. Vitamin C supplementation in pregnancy - Does it decrease rates of preterm birth? A systematic review. Am J Perinatol 2014;31:91-8.
Ugwa EA. Vitamin C supplementation in pregnancy: A review of current literature. Niger J Basic Clin Sci 2015;12:1-5.
[Table 1], [Table 2], [Table 3]
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