|Year : 2020 | Volume
| Issue : 1 | Page : 20
Relationship between Helicobacter pylori cagA genotypes infection and IL-10 and TGFβ1 genes' expression in gastric epithelial cells
Saeed Salavati1, Manouchehr Ahmadi Hedayati1, Amjad Ahmadi1, Shohreh Fakhari2, Ali Jalili2
1 Liver and Digestive Research Center, Reaserch Institute for Health Development; Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
2 Liver and Digestive Research Center, Reaserch Institute for Health Development; Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
|Date of Submission||26-Nov-2018|
|Date of Acceptance||14-Mar-2019|
|Date of Web Publication||17-Feb-2020|
Manouchehr Ahmadi Hedayati
Liver and Digestive Reaserch Center, Kurdistan University of Medical Sciences, Pasdaran, Sanandaj, 6617713446, Kurdistan
Source of Support: None, Conflict of Interest: None
Background: The correlation of Helicobacter pylori infection with gastritis, peptic ulcer, and gastric cancer has been proven. The aim of this study was to determine the effects of cagA+and cagA−genotypes of H. pylori on genes expression of interleukin (IL) -10 and tumor growth factor (TGF) β1 in gastric epithelial cells of patients with gastritis and H. pylori infection. Methods: In all, 45 gastric biopsy samples were collected from patients with gastritis and H. pylori infection admitted to Tohid Hospital in Sanandaj city. Status of urease and cagA genes of H. pylori were directly determined from the biopsy samples using polymerase chain reaction (PCR) method. Expression of IL-10 and TGF-β1 genes in gastric epithelial cells of patients with gastritis and cagA+ and cagA− genotypes of H. pylori infection was serveyed using real-time PCR method. Results: Overall, 25 samples had infection with H. pylori cagA+and 20 with cagA−genotypes. This study showed that there is a positive correlation between cagA−genotypes of H. pylori and increasing of IL-10 gene expression in gastric epithelial cells of patients with gastritis (P= 0.001). Conclusions: Level of gene expression of IL-10 as an anti-inflammatory cytokine in gastric epithelial cells of patients with H. pylori infection is connected to cagA- genotypes.
Keywords: Cytotoxin-associated gene A, gastric epithelial cells, Helicobacter pylori, interleukin-10, transforming growth factor-beta1
|How to cite this article:|
Salavati S, Hedayati MA, Ahmadi A, Fakhari S, Jalili A. Relationship between Helicobacter pylori cagA genotypes infection and IL-10 and TGFβ1 genes' expression in gastric epithelial cells. Int J Prev Med 2020;11:20
|How to cite this URL:|
Salavati S, Hedayati MA, Ahmadi A, Fakhari S, Jalili A. Relationship between Helicobacter pylori cagA genotypes infection and IL-10 and TGFβ1 genes' expression in gastric epithelial cells. Int J Prev Med [serial online] 2020 [cited 2020 Oct 24];11:20. Available from: https://www.ijpvmjournal.net/text.asp?2020/11/1/20/278487
| Introduction|| |
Helicobacter pylori is a spiral gram-negative, microaerophile with outstanding catalase characteristic and a oxidase and urease positive bacterium that is colonized inside the stomach of almost half of the world's population, and humans are known as the only natural host.H. pylori enters the stomach from the fecal–oral route and situates itself under the gastric mucosa and in contact with gastric epithelial cells. Conducted studies this subject have shown there is a connection of H. pylori infection with gastritis, gastrointestinal ulcers, and gastric cancer.
H. pylori can be colonized inside the human stomach for years and be cause inflammation in the gastric mucosa by impact on the patient's immune system. Different factors including bacterial virulence factors (such as cagA, vacA, ureA, hopQ) and host genetic factors amongst variant ethnic groups are involved in the pathogenesis of H. pylori infection. CagA that is a secretory cytotoxin reacts with the proteins inside the host cell and interferes with cellular signaling pathways through dependent and independent mechanisms of tyrosine phosphorylation. CagA protein induce the expression of inflammatory cytokines genes by activation of NF-KB at the end of the cell signaling pathways. One of the results of CagA cell signaling hijacking is the of production of neutrophil chemotaxis factors (interleukin 8) in gastric epithelial cells. Therefore CagA plays the important roles in the pathogenicity that found in 30%–70% of clinical strains. So CagA+ strains of H. pylori increase the possibility of developing gastric infection to acute gastritis, gastric atrophy, gastric ulcer, and gastric cancer.
Chronic inflammatory response, follow H. pylori infection, be causes the call of neutrophils, T and B lymphocytes and macrophages. The inflammation and tissue damage are the result of releasing inflammatory mediators, such as cytokines, oxygen free-radicals, and NO. Tumor growth factor (TGF) -β1 and IL-10 are tow inflammatory cytokines in gastric epithelial cells that follow H. pylori infection. TGF-β1 has a regulatory role in triggering of immune response against bacteria by induction of FOXP3 gene expression in CD4+ lymphocytes. IL-10 is another regulator cytokine that secreted created from gastric epithelial cells during H. pylori infection. The gastritis process upregulates FOXP3 gene expression in Treg+ cells and causes the production of inhibitory cytokines TGF-β1 and IL-10 that decreases immune system response against colonization of H. pylori in the gastric epithelial cells.
In this study, the relationship of cagA genotypes of H. pylori with TGF-β1 and IL-10 genes expression in gastric epithelial cells of patients with H. pylori infection was investigated.
| Methods|| |
A case–control study was performed including of patients with gastritis and H. pylori infection, referred to Tohid Hospitalin Sanandaj city. All gastric biopsy samples were taken by endoscopy. The gastritis with by cagA+ genotype of H. pylori were considered as case group, and biopsy samples infected by cagA− genotype of H. pylori were considered as control group. Patients with gastritis who had used aspirin or nonsteroidal anti-inflammatory drugs, or had malignancies, metabolic disorders, and immunosuppression were excluded from this study.
A total of 25 biopsy samples collected from epithelial tissues of antrum in patients with cagA+ genotype H. pylori infection and 20 biopsy samples of epithelial tissues of antrum in patients with cagA− genotype H. pylori. H. pylori infection in all biopsy samples were determined by rapid urease test (RUT). The cagA genotypes of H. pylori was determined using polymerase chain reaction (PCR) method on biopsy samples, directly after digestion and DNA extraction from tissue.
Three biopsy samples were taken from each patients with gastritis and cagA pasitive and negative H. pylori infection infected by gastritis. One of the samples was used for detection of H. pylori infection using RUT test (after 20 min, change in color of RUT solution from yellow to any color between pink and red. The second sample was put in RNAlater solution for preparation process of measurement the genes expression of TGF-β1 and IL-10 genes in gastric epithelial cells. The gastric biopsy samples were transferred to the microbiology research laboratory in Kurdistan University of Medical Sciences. The third sample was used for DNA extraction and determination of the genotype of cagA and urease gene of H. pylori.
H. pylori infection
The samples were transferred to a −70°C freezer; using DNA extraction kit (Bioflux, Japan), genomic DNA was extracted and kept in −70°C until for molecular diagnostic tests.
Afterward, confirmation of H. pylori infection in biopsy samples was performed using specific primers of urease by PCR. The sequences for urease gene primers that were designed using Gene Runner software program (Cinnagen, Iran) were F: 5′-TTCTTCCCGCTTCCACTAA-3′, R: 5′-CTGAAGTTGTCGTTATCGCC-3′. The program of PCR machine was initial denaturation 95°C, 10 min; denaturation 95°C, 30 s; annealing 52°C, 30 s; extension 72°C, 45 s; and final extension 72°C, 5 min. The PCR product length was 319 bp.
To determine of cagA virulence factor gene of H. pylori, PCR method was performed on the DNA extracted from gastric biopsy samples. Primers of cagA were designed using Gene Runner software program (Cinnagen). The sequences for cagA primers were F: 5′-GTGATGTAGGGCAAGCAGC-3′ and R: 5′-AGCTTCTGATACCGCCTGG-3′. The program of PCR machine was the same as urease gene detection exept that annealing temprature was 54 and PCR product length was 236 bp.
Extraction of total RNA from gastric biopsy tissues was done using Total RNA Extraction Kit (Pars Tous Company, Iran). The method used in this kit is based on the liquefaction power of phenol that inhibits the binding ability of RNA to borosilicate columns. The compositions of kit neutralize cellular RNase and delete DNA as well.
IL-10 and TGF-β1 genes expression in gastric epithelial cells with infection of cagA+ and cagA− genotypes of H. pylori was serveyed using relative quantitative Real Time RT PCR method. PCR efficiency (E) and cyclic threshold (CT) parameters are essential for quantitative analysis of the amounts of target genes expression compared to beta-actin gene (as the reference gene). Finaly, the amount of changes in IL-10 and TGF-β1 genes expression was calculated using the numerical values of CT and Δ CT equation.
For the accuracy and validity of real-time PCR, a standard curve drawn use four dilutions of complementary DNA including 1, 0.1, 0.01, and 0.001 and the efficiency of PCR reaction was calculated using the standard curve line slope by PCR Corbett machine (Rotor gene 6000).
Primers of IL-10, TGF-β1, and B-actin genes were designed using Gene Runner software program (v 3.05). The primers were synthesized in the lyophilized form by Bioneer company in Korea. The concentration of 100 pmol of the primers was created, and for real-time PCR, 0.1 dilution was used.
The sequences for IL-10, TGF-β1, and B-actin primers were F: 5′-AAGCCTTGTCTGAGATGATC-3′, R: 5′-AC AGGGAAGAAATCGATGAC-3′, F: 5′-AATTCCTGGC GATACCTCAG-3′, R: 5′-TAGTGAACCCGTTGATG TCC-3′, F: 5′-AGATCATTGCTCCTCCTGAG-3′, R: 5′-C TAAGTCATAGTCCGCCTAG-3′, respectively. The program of PCR machine was initial denaturation 95°C, 5 min; denaturation 95°C, 5 s; annealing 55°C, 30 s; extension 72°C, 45 s, final extension 72°C, 5 min; and melt 72–95°C, 5 min. Denaturation, annealing, and extension stages were repeated for 40 cycles.
| Results|| |
The PCR products of cagA genotypes were rounded on gel agarose 1%. The results of the study showed that from 45 biopsy samples of patients with gastritis, 25 samples have infection with cagA+ genotype of H. pylori and 20 samples have infection with cagA− genotype of H. pylori.
IL-10 and TGFβ1 genes' expression
For relative measurement of gene expression, ΔCT method was used, relative amount of expression of IL-10 and TGF-β1 genes (sample genes) was measured in comparison to B-actin gene (reference gene) using 2−ΔCT[2(ctsample − ctref)] formula.
The results of the Real Time RT PCR revealed that beta-actin gene was present in all biopsy samples. There was no significant relationship between cagA genotypes of H. pylori and TGFβ1 gene expression in gastric biopsy samples; while the expression of IL-10 cytokine was decreased in gastric epithelial cells with infection of cagA− genotype of H. pylori [Figure 1] and [Figure 2]. cagA+ genotypes were detected by PCR [Figure 3].
|Figure 1:Statistical box plot of IL-10 gene expression in cagA−and cagA+groups|
Click here to view
|Figure 2:Statistical box plot of TGF-β gene expression in cagA−and cagA+groups|
Click here to view
|Figure 3: Detection of Helicobacter pylori > and urease genes in biopsy samples by multiplex PCR|
Click here to view
The amounts of changes in IL-10 and TGF-β1 genes' expression in two groups of case (biopsy samples with infection of cagA+ genotype of H. pylori) and control (biopsy samples with infection of cagA− genotype of H. pylori) was obtained using output charts of Corbett Device (Rotor Gene 6000 Qiagen) [Figure 2] and [Figure 3]. SPSS software program (version 21.00) was used for statistical analysis of the results. Summarization and classification of data were performed by using descriptive statistics that consist of central and dispersion indicators. Analysis of date and testing the hypotheses were performed using nonparametric Mann–Whitney test. The level of statistical significance was considered 0.05 in this study.
By considering the P value obtained from Kolmogorov–Smirnov test, it can be concluded that obtained data do not follow normal distribution, and Mann–Whitney test was used for testing the above-said hypothesis. The P value obtained from Mann–Whitney test (P = 0.189) showed that there is no significant statistical difference between case and control groups in terms of TGFβ1 gene expression [Table 1]. Even though the amount of TGFβ1 gene expression in gastric epithelial cells with infection of cag+ genotype of H. pylori was more than gastric epithelial cells with infection of cag− genotype of H. pylori for 1.857, this increase in expression was not statistically significant.
|Table 1: Survey of IL-10 and TGF-β genes' expression by nonparametric Mann–Whitney test in two groups of patients with gastritis and H. pylori cagA− and cagA+ genotypes infection|
Click here to view
Prior to performing the test related to the above-said hypothesis, first, a normality test (Kolmogorov–Smirnov) was performed for investigating data distribution. The results showed that there is a meaningful connection between cagA+ genotype of H. pylori and IL-10 gene expression among gastric biopsy samples of patients with gastritis [Table 1]. With regard to the P value obtained from Kolmogorov–Smirnov test, it could be concluded that the obtained data do not follow normal distribution; therefore, Mann–Whitney nonparametric test was used for testing the above-mentioned hypothesis [Table 1]. The P value obtained from Mann–Whitney test (P = 0.001) revealed a significant difference between two groups of case and control in terms of IL-10 gene expression. The amount of IL-10 gene expression in biopsy samples of gastric epithelial cells of patients with gastritis and infection of cagA− genotype of H. pylori was increased 3.073 times the gastric epithelial cells of patients with gastritis and infection of cagA+ genotype of H. pylori; and this increase in expression is statistically significant. Standard deviation of the results of IL-10 gene expression in the case group was high, while standard deviation of the results of IL-10 gene expression in the control group was low (P = 0.001) [Table 1].
| Discussion|| |
While H. pylori is colonized in the stomach of half the world's population, there are different information with regard to the prevalence of H. pylori in Iran; however, it is estimated that the prevalence rate of H. pylori in various regions of Iran is between 50% and 90%., This bacterium causes colonization in the gastric mucosal tissue, damage of epithelium cells, and long-term chronic inflammation by the aid of certain virulence factors such as surface proteins, and it seems like gastric inflammation and immune response to infection are the basis of creation of disease resulting from it. Colonization of H. pylori in stomach is due to gastritis. Despite gastritis, due to unknown reasons, gastric cancer and peptic ulcers are seen in a low number of patients with H. pylori infection. However, a series of factors such as the host's immune system response against H. pylori, environmental factors, and different genotypes of H. pylori all play a role in creation of gastric cancer and peptic ulcers.
Some genotypes of H. pylori are more success in colonization that CagA as an important virulence factor is noteworthy. In this regard, the role of pathogenicity factors such as cagA and vacA has been studied in numerous instances and the role of these cytokines in the development of some disruptions in the way of apoptosis and cancerization of gastric epithelial cells has been proved.CagA protein can affect the colonization process of bacteria indirectly and by the effect of secretion of cytokines from gastric epithelial cells. Connection of cagA pathogenic factor with pathological factors in gastric epithelial cells shows that the level of inflammation, neutrophilic inflammation, damage of gastric epithelial cells, and also increase in the risk of gastric atrophy and intestinal metaplasia are significantly higher in patients with infection of cagA+ strains in comparison to patients with infection of cagA− strains. Nevertheless, statistical analyses of some studies such as the study by Razavi et al. show that the amount of chronic inflammation, neutrophilic inflammation, and the level of colonization of H. pylori in patients with infection of cagA+ was significantly higher compared with patients with infection of cagA−. In addition, the results of the study by Razavi et al. showed that the prevalence of Foxp3+ cells and IL-10 and TGF-β1 cytokines in patients with infection of H. pylori was significantly higher in comparison to patients without infection of H. pylori. In that study, in which the connection of pathogenic factor of cagA with the amount of TGF-β1 gene expression was studied, it was determined that there is no significant relationship between the two (P = 0.189 > 0.05), which is compatible with the results of the present research. Previous studies show that H. pylori stimulates a response in Th1, Th17, and Treg cells. Other studies as well have proven the role of Treg cells in immune response of the host against H. pylori infection and have shown that CD4+ C25+ Treg cells have a very important role in suppression of host's immune system.
These studies showed that after antibiotic treatment and eradication of H. pylori infection, activity of Treg cells and gene expression of TGF-β1 and IL-10 shall considerably decrease in gastric mucosa, which is the result of negative feedback of H. pylori infection in the expression of these cytokines. In the studies conducted by Robinson et al., a comparison was performed between two groups of patients with infection of H. pylori, with and without the existence of gastric ulcer, and it was revealed that the prevalence of (IL-10+) Treg cells in patients suffering from gastric ulcer with infection of H. pylori was lower compared with infected patients who did not have gastric ulcer. Furthermore, the level of IL-10 expression has a direct relationship with the amount of bacterial colonization. IL-10 gene expression in gastric epithelial cells of patients with infection of cagA+ genotype of H. pylori was more than the gastric epithelial cells of patients with infection of cagA− genotype of H. pylori, while no significant connection was observed between the prevalence of CD4+CD25hi cells and cagA genotypes. A study performed by Hernandez et al. concluded that IL-10 cytokine expression in gastric epithelial cells of children with infection of H. pylori was higher compared with gastric epithelial cells of children free of infection.
In addition, no significant relationship was observed in the amount of IL-10 and TGF-β cytokines in children's gastric epithelial cells with H. pylori infection, with or without the existence of peptic ulcer. In this study, which was performed on gastric biopsy samples of patients suffering from gastritis, it was determined that there is a significant relationship between IL-10 gene expression and cagA pathogenic factor (P = 0.001 < 0.05), and IL-10 gene expression in gastric epithelial cells of patients with cagA+ genotypes of H. pylori is less than gastric epithelial cells of patients infected by cagA− genotype of H. pylori. These results show the ability of cagA+ strains of H. pylori in colonization that follows the anti-inflammatory effect of IL-10. H. pylori infection has a high rate or prevalence in today's societies which, if not treated, results in inflammation and increase in cytokine production. Pathogenicity factors of H. pylori such as cagA in patients are researchable, and it was determined in this study that there is no significant relationship between pathogenicity factor of cagA and TGFβ1 gene expression; however, there is in fact a significant relationship between pathogenicity factor of cagA and IL-10 gene expression, and IL-10 gene expression is found more in cagA− than cagA+.
As a conclusion, the chance of bacterial colonization and creation of mild and persistent infections such as gastritis increase by the cagA− strains of H. pylori cause the increase in IL-10 anti-inflammatory cytokine expression and suppression of immune system.
Financial support and sponsorship
This work was supported by a Grant-in-Aid for obtaining Master's Degree of Mr. Saeed salavati in the field of Microbiology from Kurdistan University of Medical Sciences. We are grateful Dr. Roghayeh Ghadyani.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Moosazadeh M, Lankarani KB, Afshari M. Meta-analysis of the prevalence of Helicobacter pylori
infection among children and adults of Iran. Int J Prev Med 2016;7:48.
Dunne C, Dolan B, Clyne M. Factors that mediate colonization of the human stomach by Helicobacter pylori
. World J Gastroenterol 2014;20:5610-24.
Chichaklu AH, Hedayati MA, Esmaili FS, Ghaderi E, Shiri MHD. Prevalence of Hopqalleles and relationship between cagA
S1 with hopQI
gene in Helicobacter Pylori
strains isolated from patients with peptic ulcer referred to Towhid Hospital in Sanandaj (2014). Biosci Biotech Res Asia 2016;13:91-4.
Hatakeyama M. Helicobacter pylori
CagA and gastric cancer: A paradigm for hit-and-run carcinogenesis. Cell Host Microbe 2014;15:306-16.
Yong X, Tang B, Li B, Xie R, Hu CJ, Luo G, et al
. Helicobacter pylori
virulence factor CagA promotes tumorigenesis of gastric cancer via multiple signaling pathways. Cell Commun Signal 2015;13:30.
Lai CH, Wang HJ, Chang YC, Hsieh WC, Lin HJ, Tang CH, et al
. Helicobacter pylori
CagA-mediated IL-8 induction in gastric epithelial cells is cholesterol-dependent and requires the C-terminal tyrosine phosphorylation-containing domain. FEMS Microbiol Lett 2011;323:155-63.
Razavi A, BagheriN, Dehkordi FA, Shirzad M, RahimianG, Rafieian-Kopaei M,et al
. Comparative immune response in children and adults with H. pylori
infection. J Immunol Res 2015;2015:315957.
Zandi S, Hedayati MA, Mohammadi E, Sheikhesmaeili F. Helicobacter pylori
infection increases sirt2 gene expression in gastric epithelial cells of gastritis patients. Microb Pathog 2018;20:120-3.
Schmittgen TD, Livak KJ. Analyzing real- time PCR data by the comparative CT method. Nat Protoc 2008;6:1101-8.
Eshraghian A. Epidemiology of Helicobacter pylori
infection among the healthy population in Iran and countries of the Eastern Mediterranean region: A systematic review of prevalence and risk factors. World J Gastroenterol 2014;20:17618.
Saravle SM, Hedayati MA, Mohammadi E, Sheikhesmaeili F, Nikkhou B. Sirt1 gene expression and gastric epithelial cells tumor stage in patients with Helicobacter pylori
infection. Asian Pac J Cancer Prev 2018;19:913-6.
Rad R, Brenner L, Bauer S, Schwendy S, Layland L, da Costa CP, et al
. CD25+/Foxp3+T cells regulate gastric inflammation and Helicobacter pylori
colonization in vivo
. Gastroenterology 2006;131:525-37.
Robinson K, Kenefeck R, Pidgeon EL, Shakib S, Patel S, Polson RJ, et al
. Helicobacter pylori
-induced peptic ulcer disease is associated with inadequate regulatory T cell responses. Gut 2008;57:1375-85.
Hernandez C, Serrano C, Einisman H, Villagrán A, Peña A, Duarte I, et al
. Peptic ulcer disease in Helicobacter pylori
-infected children: Clinical findings and mucosal immune response. J Pediatr
Gastroenterol Nutr 2014;59:773-8.
[Figure 1], [Figure 2], [Figure 3]