Background: The ABILOCO is a questionnaire assessing and measuring the locomotion ability to perform activities daily living from easy to hard in stroke patients. Methods: The ABILOCO was translated into Persian and retranslated into English. Forty hemiplegic stroke patients with an average age (60 ± 7.5 years) participated in the study. The participants were interviewed using ABILOCO and were assessed by the interviewer and observer. The total logit score of the participants was also assessed by using the interclass correlation coefficient (ICC). Results: The results were showed the high correlation between the first interview of interviewer and the observer (ICC = 0.8, P < 0.05). In addition, a strong relationship was observed between the interviewer's first and second interviews after a week (ICC = 0.85, P < 0.05). Conclusions: The Persian edition of ABILOCO is reliable to assess walking ability of stroke patients in clinics and studies.