International Journal of Preventive Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 10  |  Issue : 1  |  Page : 45-

Evaluating the protective role of ascorbic acid in malathion-induced testis tissue toxicity of male rats


Faezeh Ghorbani Taherdehi1, Mohammad Reza Nikravesh1, Mahdi Jalali1, Alireza Fazel2, Mahmoud Gorji Valokola3 
1 Department of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, Iran
2 Microanatomy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Pharmacodynamics and Toxicology, Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence Address:
Mohammad Reza Nikravesh
Department of Anatomy and Cell Biology, School of Medicine, Azadi Square, Mashhad University of Medical Sciences, Mashhad
Iran

Background: Malathion is one of organophosphate pesticides that is widely used in agriculture and crops to control insects. Malathion affects body organs such as the reproductive system by inhibiting acetylcholinesterase activity and induction of oxidative stress. This study is aimed to investigate the effects of malathion on glutathione (GSH) and malondialdehyde (MDA) level in testis of male rats, as well as to study the protective role of Ascorbic Acid. Methods: In this study, 30 adult male Wistar rats weighing approximately 200–250 g were divided into 5 groups of 6 rats each. These groups include a control group (no intervention), sham (normal saline 0.9%), experimental Group 1 (malathion 50 mg/kg), experimental Group 2 (Malathion 50 mg/kg + Ascorbic Acid 200 mg/kg), and experimental Group 3 (Ascorbic Acid 200 mg/kg). Malathion, solvents, and ascorbic acid were injected intraperitoneally. After 6 weeks, all groups were anesthetized, and the right testis was used to measure levels of MDA and GSH. MDA as a marker of lipid peroxidation and GSH content was used. Results: The results showed that malathion increased MDA level and decreased GSH level compared with the control group (P < 0.001). It was also found that administration of malathion in combination with ascorbic acid reduced MDA level and increased the GSH level. Conclusions: Malathion-induced lipid peroxidation and oxidative stress in the testis of rats. In addition, it seems that ascorbic acid, due to its antioxidant capabilities, can improve malathion-induced poisonous changes.


How to cite this article:
Taherdehi FG, Nikravesh MR, Jalali M, Fazel A, Valokola MG. Evaluating the protective role of ascorbic acid in malathion-induced testis tissue toxicity of male rats.Int J Prev Med 2019;10:45-45


How to cite this URL:
Taherdehi FG, Nikravesh MR, Jalali M, Fazel A, Valokola MG. Evaluating the protective role of ascorbic acid in malathion-induced testis tissue toxicity of male rats. Int J Prev Med [serial online] 2019 [cited 2021 Apr 22 ];10:45-45
Available from: https://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2019;volume=10;issue=1;spage=45;epage=45;aulast=Taherdehi;type=0