International Journal of Preventive Medicine

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 11  |  Issue : 1  |  Page : 122-

The effect of hydro-alcoholic extract ofRheum Turkestanicum Roots against oxidative stress in endothelial cells


Azar Hosseini1, Sahar Sheikh3, Mohammad Soukhtanloo2, Bizhan Malaekeh-Nikouei3, Arezoo Rajabian4 
1 Pharmacological Research Center of Medicinal Plants; Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2 Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Nanotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran
4 Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence Address:
Arezoo Rajabian
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad
Iran

Introduction: Cardiovascular disorders (CVD) are a common cause of mortality worldwide. Oxidative stress is thought to be a major factor leading to CVD. Anti-oxidants such as medicinal plants may have a role in the mitigation of vascular problems through free radicals scavenging. In this study, we evaluated the protective effects of Rheum turkestanicum against hydrogen peroxide (H2O2)-induced toxicity in endothelial cells (BAE-1). Methods: To evaluate the protective effect of R. turkestanicum against H2O2toxicity, four groups comprised of control group (the cells without any treatment), H2O2group (the cells incubated with H2O2 (200 μM)), and treatment groups (the cells treated with R. turkestanicum (12200 μg/ml) alone or 24h before exposure to H2O2). Quercetin (30.23 μg/ml) was used as a bioactive ingredient of the extract. Then the cell viability, reactive oxygen species, lipid peroxidation, and apoptosis were evaluated. Results: H2O2exposure reduced cell viability to 13.6 ± 1.6%, enhanced ROS generation to 1445 ± 80.7%, lipid peroxidation (LPO, 290 ± 13% of control), and apoptotic cells (P < 0.001). In contrast, compared with H2O2 group, R. turkestanicum and quercetin significantly restored the cell viability to 80.3 ± 1.6 and 87.2 ± 2.1%, ROS formation to 186 ± 10 and 129 ± 1%, as well as LPO to 130.7 ± 7.7 and 116 ± 2.5 of control, respectively (P < 0.001). Therefore, the extract reduced H2O2-induced toxicity in BAE-1 cells by scavenging of free radicals. Conclusion:Our findings demonstrated that the extract might reduce toxicity of endothelial cells by attenuation of oxidative stress, which can be related to the presence of active ingredients including quercetin.


How to cite this article:
Hosseini A, Sheikh S, Soukhtanloo M, Malaekeh-Nikouei B, Rajabian A. The effect of hydro-alcoholic extract ofRheum Turkestanicum Roots against oxidative stress in endothelial cells.Int J Prev Med 2020;11:122-122


How to cite this URL:
Hosseini A, Sheikh S, Soukhtanloo M, Malaekeh-Nikouei B, Rajabian A. The effect of hydro-alcoholic extract ofRheum Turkestanicum Roots against oxidative stress in endothelial cells. Int J Prev Med [serial online] 2020 [cited 2022 Oct 1 ];11:122-122
Available from: https://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2020;volume=11;issue=1;spage=122;epage=122;aulast=Hosseini;type=0